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Confocal microscopes employ one or more types of lasers to obtain the wavelengths required for fluorochrome excitation, the most common wavelengths being 365, 488, 515, 568, 633, and 648 nm (see Table 12-1). The laser beam is expanded by a lens to fill the back aperture of the objective to give the minimum-size diffraction spot on the specimen. Because the back aperture must be uniformly illuminated across its diameter, and because the light intensity profile across a laser beam is Gaussian, the beam is expanded considerably so that the portion filling the lens is more uniform. The power of lasers fitted by manufacturers for confocal illumination (range, 5 50 mW) is chosen based on the factor of light loss from beam spreading and the intensity of light required to give close to saturating excitation of the appropriate fluorochromes in the focused beam in the specimen. Laser power is adjusted using the laser s power-control dial, or additionally with an acousto-optical tunable filter (AOTF). For lasers that emit at several wavelengths, the AOTF can also be used to select a specific laser line for excitation. Normally lasers are operated at their midpower range during acquisition to prolong the lifetime of the laser. Many lasers can also be placed in a standby (low-power) mode, at which the amplitude of laser light is minimal. In cases where specimens are subject to photobleaching or biological damage, laser power is reduced to a point where fluorescence emission is still adequate for acquiring an image. Since the intensity of the focused laser beam can damage the eye, confocal systems contain an interlock device that prevents the operator from seeing the laser through the eyepieces during laser scanning or during visual inspection of the sample with a mercury arc lamp.

with probability 1,

DNA ploidy studies are recommended for problematic cases, as approximately 99% of partial moles show a triploid DNA content with the remaining 1% showing a tetraploid DNA content When evaluating DNA content, it is important that the pathologist select a test block containing abundant chorionic villi rather than abundant decidua The recognition and diagnosis of complete moles is typically more straightforward than with partial moles, but a de nitive diagnosis of early complete mole still represents a challenge for the pathologist In most cases, the diagnosis can be made on routine histology For particularly problematic cases, immunohistochemistry for p57 can be performed This stain is positive when a maternal allele is present Complete moles do not have maternal chromosomes, resulting in complete loss of p57 expression in chorionic villi trophoblasts.

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Scripts attached to movie clips or buttons execute when an event occurs. An event is an occurrence in the movie such as a mouse movement, a keypress, or a movie clip being loaded. You can use ActionScript to find out when these events occur and execute specific scripts depending on the event. Actions attached to a button or movie clip are enclosed in special actions called handlers. The onClipEvent and on actions are called handlers because they handle or manage an event. You can specify one or more events for each handler. Movie clip and button actions execute when the event specified by the handler occurs. You can attach more than one handler to an object if you want different actions to execute when different events occur.

studied in Et4NClO4-acetonitrile (AN) [5, 6]. 3) In this method, the substance to be measured is generated in situ by controlled-potential electrolysis and its concentration at every moment is traced. Thus, the reactivity of an unstable substance (lifetime of * 100 ls) can be studied. Various other methods have been developed for studying rapid reactions, e.g. the pulse radiolysis and flash photolysis methods. Compared with such methods, the present method has a merit that the reactive substance can be generated at the best condition by the controlled-potential electrolysis. However, the cell in Fig. 9.2(b) has a disadvantage in that the concentration of the electrogenerated substance decreases with increasing distance from the OTE surface. Because of this, simulation of the reaction is very difficult, except for the first order (or pseudo-first-order) reactions. For more complicated reactions, it is desirable that the concentration of the electrogenerated species is kept uniform in the solution. With a thin-layer cell, a solution of uniform concentration can be obtained by complete electrolysis, but it takes * 30 s. Thus, the thin-layer cell is applicable only for slow reactions. For faster reactions, a column-type cell for rapid electrolysis is convenient. Okazaki et al. [7] constructed a stopped-flow optical absorption cell using one or two column-type cells (Fig. 9.4) and used it to study the dimerization of the radical cations (TPA +) of triphenylamine and the reactions of the radical cation (DPA +) of 9,10-diphenylanthracene with water and alcohols. Using the stopped-flow cell, reactions of substances with a half-life of * 1 s can be studied in solutions of uniform concentrations.

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TABLE 1.3 Descriptive Summary of the Weight-Loss Data: Means (Y) and Standard Deviations (S) Mean Standard Deviation Y 1 = 8.7 S 1 = 3.8 Y 2 = 11.7 S 2 = 4.6

Pathologists should ideally select a block that contains both chorionic villi and decidua as the decidua cells serve as positive internal controls Placental site nodules (PSNs) are small, circumscribed foci of hyalinized implantation site containing intermediate trophoblasts typically identi ed in endometrial biopsies/curettings These are benign lesions that typically occur in women of reproductive age, although pregnancy history.

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